Visualization of centrioles and basal bodies by fluorescent staining with nonimmune rabbit sera.

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Visualization of centrioles and basal bodies by fluorescent staining with nonimmune rabbit sera

Several sera from nonimmunized rabbits have been found which stain centrioles and basal bodies by indirect immunofluorescence in a wide variety of cell types. So far, approximately 10% of the rabbit sera that we have examined gave strong positive staining of centrioles and basal bodies. Cytoplasmic networks, mitotic spindles, and ciliary axonemes, however, remain unstained. This specific fluore...

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Nonimmune Fluorescent Protein Staining of Neoplasms

In 1952 Weiler demonstrated with ultraviolet microscopy that anti-rat liver microsomal and mitochondrial rabbit globulin conjugated with fluorescein isocyanate became selectively attached to normal and hyperplastic rat liver but not to paradimethylamino-azobenzol-induced hepato-carcinoma within such livers. In 1956 he demonstrated similar antigenic differences between normal hamster kidney and ...

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Basal Bodies, but Not Centrioles, in Naegleria

Amebae of Naegleria gruberi transform into flagellates whose basal bodies have the typical centriole-like structure. The amebae appear to lack any homologous structure, even during mitosis. Basal bodies are constructed during transformation and, in cells transforming synchronously at 25 degrees C, they are first seen about 10 min before flagella are seen. No structural precursor for these basal...

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We have localized a fraction of the enzyme, purine nucleoside phosphorylase (PNP), to the centrioles and basal bodies of mammalian, avian, and protozoan cells. Two completely independent methods were used, one based on the ultrastructural cytochemistry of the enzyme activity and one based on immunofluorescence microscopy using an antibody raised in rabbit against purified human PNP. PNP catalyz...

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The Centrioles, Centrosomes, Basal Bodies, and Cilia of Drosophila melanogaster

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1978

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.79.2.526